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c di gmp levels  (ATCC)


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    ATCC c di gmp levels
    Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
    C Di Gmp Levels, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 23820 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c di gmp levels/product/ATCC
    Average 99 stars, based on 23820 article reviews
    c di gmp levels - by Bioz Stars, 2026-03
    99/100 stars

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    1) Product Images from "Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module"

    Article Title: Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.111086

    Enhanced Psl production at RT was mediated by the c-di-GMP–FleQ pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
    Figure Legend Snippet: Enhanced Psl production at RT was mediated by the c-di-GMP–FleQ pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.

    Techniques Used: Activity Assay, Mutagenesis

    The DGC SiaD is primarily responsible for the elevated c-di-GMP content and enhanced Psl production at RT in ATCC 27853. A , relative maximal biofilm biomass of 28 isogenic mutants relative to the ATCC 27853 WT at RT. Blue , genes identified in RNA-seq analysis. B , relative maximal biofilm formation of ATCC 27853 and its isogenic Δ siaD strain at RT and 37 °C. C , relative maximal biofilm formation of ATCC 27853, isogenic Δ siaD mutant, and Δ siaD mutant complemented with rhamnose-inducible expression of siaD at 48 h at RT. VC, the pJM253 vector control. D – F , relative activity of the chromosomal P psl - lux transcriptional fusion reporter ( D ), Psl level in biofilm matrix ( E ), and intracellular c-di-GMP level ( F ) in ATCC 27853 and its isogenic Δ siaD mutant at RT. Midlog phase static cells harvested at 24 h at RT were collected for the analysis in ( C–F ). Data are represented as mean ± SD. (A–E), n = 3 independent experiments. ( F ), n = 2 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.
    Figure Legend Snippet: The DGC SiaD is primarily responsible for the elevated c-di-GMP content and enhanced Psl production at RT in ATCC 27853. A , relative maximal biofilm biomass of 28 isogenic mutants relative to the ATCC 27853 WT at RT. Blue , genes identified in RNA-seq analysis. B , relative maximal biofilm formation of ATCC 27853 and its isogenic Δ siaD strain at RT and 37 °C. C , relative maximal biofilm formation of ATCC 27853, isogenic Δ siaD mutant, and Δ siaD mutant complemented with rhamnose-inducible expression of siaD at 48 h at RT. VC, the pJM253 vector control. D – F , relative activity of the chromosomal P psl - lux transcriptional fusion reporter ( D ), Psl level in biofilm matrix ( E ), and intracellular c-di-GMP level ( F ) in ATCC 27853 and its isogenic Δ siaD mutant at RT. Midlog phase static cells harvested at 24 h at RT were collected for the analysis in ( C–F ). Data are represented as mean ± SD. (A–E), n = 3 independent experiments. ( F ), n = 2 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

    Techniques Used: RNA Sequencing, Mutagenesis, Expressing, Plasmid Preparation, Control, Activity Assay

    A model to illustrate the activation of SiaABCD and its mediated biofilm enhancement in ATCC 27853 in response to temperature downshifts. Temperature downshift from 37 °C to RT causes membrane perturbations in ATCC 27853, which induce SiaA phosphatase activity to promote the formation of SiaC–SiaD complex and DGC activity of SiaD. The activated Sia system regulates psl production via c-di-GMP–FleQ–Psl pathway. Increased Psl production enhanced biofilm formation of ATCC 27853 at RT through enhancing both surface attachment and biofilm matrix development. c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.
    Figure Legend Snippet: A model to illustrate the activation of SiaABCD and its mediated biofilm enhancement in ATCC 27853 in response to temperature downshifts. Temperature downshift from 37 °C to RT causes membrane perturbations in ATCC 27853, which induce SiaA phosphatase activity to promote the formation of SiaC–SiaD complex and DGC activity of SiaD. The activated Sia system regulates psl production via c-di-GMP–FleQ–Psl pathway. Increased Psl production enhanced biofilm formation of ATCC 27853 at RT through enhancing both surface attachment and biofilm matrix development. c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

    Techniques Used: Activation Assay, Membrane, Activity Assay



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    c di gmp levels  (ATCC)
    99
    ATCC c di gmp levels
    Enhanced Psl production at RT was mediated by the <t>c-di-GMP–FleQ</t> pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.
    C Di Gmp Levels, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c di gmp levels/product/ATCC
    Average 99 stars, based on 1 article reviews
    c di gmp levels - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

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    Enhanced Psl production at RT was mediated by the c-di-GMP–FleQ pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.

    Journal: The Journal of Biological Chemistry

    Article Title: Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module

    doi: 10.1016/j.jbc.2025.111086

    Figure Lengend Snippet: Enhanced Psl production at RT was mediated by the c-di-GMP–FleQ pathway. A , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ fleQ , Δ rpoS , and Δ amrZ mutants at RT. Data are represented as mean ± SD, n = 3 independent experiments. B , relative activity of the chromosomal P psl - lux transcriptional fusion reporter collected at half maximal biofilm in ATCC 27853 and its isogenic Δ fleQ mutant at RT. Data are represented as mean ± SD, n = 3 independent experiments. C , intracellular c-di-GMP level at half maximal biofilm culture of ATCC 27853 at RT and 37 °C. Data are represented as mean ± SD, n = 2 independent experiments. D , dynamic biofilm formation of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. E , relative maximal biofilm biomass of ATCC 27853 and its isogenic Δ wspF strain at RT and 37 °C. Data are represented as mean ± SD, n = 3 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate.

    Article Snippet: LC-MS/MS revealed a reduction in c-di-GMP levels in ATCC 27853 Δ siaD cells compared to the WT ( F ).

    Techniques: Activity Assay, Mutagenesis

    The DGC SiaD is primarily responsible for the elevated c-di-GMP content and enhanced Psl production at RT in ATCC 27853. A , relative maximal biofilm biomass of 28 isogenic mutants relative to the ATCC 27853 WT at RT. Blue , genes identified in RNA-seq analysis. B , relative maximal biofilm formation of ATCC 27853 and its isogenic Δ siaD strain at RT and 37 °C. C , relative maximal biofilm formation of ATCC 27853, isogenic Δ siaD mutant, and Δ siaD mutant complemented with rhamnose-inducible expression of siaD at 48 h at RT. VC, the pJM253 vector control. D – F , relative activity of the chromosomal P psl - lux transcriptional fusion reporter ( D ), Psl level in biofilm matrix ( E ), and intracellular c-di-GMP level ( F ) in ATCC 27853 and its isogenic Δ siaD mutant at RT. Midlog phase static cells harvested at 24 h at RT were collected for the analysis in ( C–F ). Data are represented as mean ± SD. (A–E), n = 3 independent experiments. ( F ), n = 2 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

    Journal: The Journal of Biological Chemistry

    Article Title: Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module

    doi: 10.1016/j.jbc.2025.111086

    Figure Lengend Snippet: The DGC SiaD is primarily responsible for the elevated c-di-GMP content and enhanced Psl production at RT in ATCC 27853. A , relative maximal biofilm biomass of 28 isogenic mutants relative to the ATCC 27853 WT at RT. Blue , genes identified in RNA-seq analysis. B , relative maximal biofilm formation of ATCC 27853 and its isogenic Δ siaD strain at RT and 37 °C. C , relative maximal biofilm formation of ATCC 27853, isogenic Δ siaD mutant, and Δ siaD mutant complemented with rhamnose-inducible expression of siaD at 48 h at RT. VC, the pJM253 vector control. D – F , relative activity of the chromosomal P psl - lux transcriptional fusion reporter ( D ), Psl level in biofilm matrix ( E ), and intracellular c-di-GMP level ( F ) in ATCC 27853 and its isogenic Δ siaD mutant at RT. Midlog phase static cells harvested at 24 h at RT were collected for the analysis in ( C–F ). Data are represented as mean ± SD. (A–E), n = 3 independent experiments. ( F ), n = 2 independent experiments. Asterisks indicate statistical significance. ∗, p < 0.05. ∗∗∗, p < 0.01. ns, not significant (based on Student’s t test). c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

    Article Snippet: LC-MS/MS revealed a reduction in c-di-GMP levels in ATCC 27853 Δ siaD cells compared to the WT ( F ).

    Techniques: RNA Sequencing, Mutagenesis, Expressing, Plasmid Preparation, Control, Activity Assay

    A model to illustrate the activation of SiaABCD and its mediated biofilm enhancement in ATCC 27853 in response to temperature downshifts. Temperature downshift from 37 °C to RT causes membrane perturbations in ATCC 27853, which induce SiaA phosphatase activity to promote the formation of SiaC–SiaD complex and DGC activity of SiaD. The activated Sia system regulates psl production via c-di-GMP–FleQ–Psl pathway. Increased Psl production enhanced biofilm formation of ATCC 27853 at RT through enhancing both surface attachment and biofilm matrix development. c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

    Journal: The Journal of Biological Chemistry

    Article Title: Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module

    doi: 10.1016/j.jbc.2025.111086

    Figure Lengend Snippet: A model to illustrate the activation of SiaABCD and its mediated biofilm enhancement in ATCC 27853 in response to temperature downshifts. Temperature downshift from 37 °C to RT causes membrane perturbations in ATCC 27853, which induce SiaA phosphatase activity to promote the formation of SiaC–SiaD complex and DGC activity of SiaD. The activated Sia system regulates psl production via c-di-GMP–FleQ–Psl pathway. Increased Psl production enhanced biofilm formation of ATCC 27853 at RT through enhancing both surface attachment and biofilm matrix development. c-di-GMP, cyclic di-guanosine 3′,5′-monophosphate; DGC, diguanylate cyclase.

    Article Snippet: LC-MS/MS revealed a reduction in c-di-GMP levels in ATCC 27853 Δ siaD cells compared to the WT ( F ).

    Techniques: Activation Assay, Membrane, Activity Assay